Induced pluripotent stem cells (iPSCs) is considered as a breakthrough in regenerative medicine. However, the in vitro proliferation and determining its differentiation is still challenging.
In this project, we aim to develop a novel scaffold for the in vitro proliferation and differentiation of human induced pluripotent stem cells (hiPSCs) into bone cells. The scaffold will be fabricated using a combination of biocompatible materials including gelatin, β-tricalcium phosphate (β-TCP), poly lactic acid (PLA) nanofibers, and gold nanoparticles (AuNPs). Gelatin will serve as the base scaffold structure, providing a biocompatible and degradable property. PLA nanofibers will be incorporated to generate piezoelectric properties, the piezoelectric concept is known to stimulate stem cell proliferation and differentiation into bone tissue. Gold nanoparticles will enhance the electrical conductivity and promote cell-scaffold interactions. The addition of β-TCP, a bioceramic with bone-like properties, will further mimic the natural bone environment. This multi-functional scaffold will be investigated for its ability to support hiPS cells differentiation into bone cells, ultimately paving the way for potential advancements in regenerative medicine.
