Induced pluripotent stem cells (iPSCs) are considered a breakthrough in regenerative medicine. However, determining the in vitro proliferation and its differentiation is still challenging. In this project, we aim to develop a novel scaffold for the in vitro proliferation and differentiation of human induced pluripotent stem cells (hiPSCs) into bone cells. The scaffold will be fabricated using a combination of biocompatible materials, including gelatin, β-tricalcium phosphate (β-TCP), poly lactic acid (PLA) nanofibers, and gold nanoparticles (AuNPs). Gelatin will serve as the base scaffold structure, providing a biocompatible and degradable property.
PLA nanofibers will be incorporated to generate piezoelectric properties; the piezoelectric concept is known to stimulate stem cell proliferation and differentiation into bone tissue. Gold nanoparticles will enhance the electrical conductivity and promote cell-scaffold interactions. The addition of β-TCP, a bioceramic with bone-like properties, will further mimic the natural bone environment. This multi-functional scaffold will be investigated for its ability to support hiPS cells differentiation into bone cells, ultimately paving the way for potential advancements in regenerative medicine.
